Expression, crystallization and preliminary X-ray diffraction studies of thermostable β-1,3-xylanase fromThermotoga neapolitanastrain DSM 4359
نویسندگان
چکیده
منابع مشابه
Preliminary X-ray diffraction analysis of thermostable β-1,4-xylanase from Streptomyces sp. S9.
Xylanase, which catalyzes the random hydrolysis of internal xylosidic linkages, is a critical enzyme participating in xylan decomposition and has been widely applied in industrial utilizations. Xylanase isolated from the extremophilic Streptomyces sp. S9 (XynAS9) possesses broad adaptability to temperature and pH and thus is an attractive candidate in industrial applications. In particular, the...
متن کاملCrystallization and preliminary X-ray diffraction studies of soybean agglutinin.
Soybean agglutinin crystallizes in the monoclinic space group C2 with unit cell dimensions a = 118.6 A, b = 88.9 A, c = 165.9 A, beta = 103.0 degrees and one tetramer of 120,000 Mr per asymmetric unit. The crystals are suitable for high-resolution work.
متن کاملProinsulin: Crystallization and preliminary x-ray diffraction studies.
Bovine proinsulin has been crystallized under a variety of conditions at both neutral and acid pH. Microtechniques were employed with sample weights of about 200 mug and volumes of 5-20 mul. The crystalline preparations all differ from each other morphologically.X-ray photographs of one form, tetragonal bipyramids grown at pH 3 with added ammonium sulphate solution, established the space group ...
متن کاملCrystallization and preliminary X-ray diffraction
The amyloidogenic Leu55Pro variant of transthyretin has been expressed, purified and crystallized in space group C2. The cell constants are a--149.99, b = 78.74, c = 98.95A, = 100.5 ° and the crystals diffract to 2.7,~ resolution. There are eight monomers in the asymmetric unit giving a V M = 2.6,~,3 Da -w and 53% solvent content. In the wild-type protein, the crystals are orthorhombic with two...
متن کاملPurification, crystallization and preliminary X-ray diffraction analysis of thermostable nitrile hydratase
Introduction Nitrile-metabolizing enzyme systems are present in a variety of microorganisms from diverse ecosystems. These enzymes are of interest for the industrial production of amides and carboxylic acids. The nitrile hydratases (NHase; EC 4.2.1.84) catalyse the conversion of nitriles to corresponding amides. The amides are then converted to carboxylic acids by amidase (recently reviewed in ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Acta Crystallographica Section F Structural Biology and Crystallization Communications
سال: 2011
ISSN: 1744-3091
DOI: 10.1107/s1744309111017222